Preparation and Characterization of Superparamagnetic Iron Oxide Nanoparticles (Fe3O4) for Biological Applications

Superparamagnetic iron oxide nanoparticles (SPIONs) are developed considering the importance of this class in different fields biochemical and biomedical applications owing to their distinctive chemical physical properties. In work, preparation (SPIONs (using Co-precipitation method has been done as most commonly used wet magnetic for biological applications. The SPIONs synthesis was based on sodium hydroxide (NaOH) mediated precipitation Fe3+ Fe2+ salts an aqueous solution using trisodium citrate surfactant within a closed system. size stability magnetite were carefully controlled parameters order obtain with small particle distribution that is needed synthesized Fe3O4 characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), transmission (TEM), vibrating sample magnetometer (VSM), Zeta potential analysis (Zp). XRD pattern showed presence peaks corresponding phase Fe3O4. Moreover, SEM TEM results revealed spherical particles mean diameter ≥ 5 nm. monodispersed successfully prepared hydrodynamic 209.32 nm at stirring speed 900 rpm NaOH concentration 1.2 gm. considerably dependent rate concentration. exhibited superparamagnetic behavior saturation magnetization around 50 emu/g.